coli by building a site-specific genomic recombination-mediated cassette exchange (RMCE) system that allows for the facile construction and testing of large libraries of genetic designs integrated into precise genomic locations. Here we develop a novel multiplexed assay to study promoter function in E.
Despite our knowledge of these motifs, it is still not possible to predict the strength and regulation of a promoter from primary sequence alone. coli, decades of studies have revealed most, if not all, of the sequence elements necessary to encode promoter function. Promoters are the key drivers of gene expression and are largely responsible for the regulation of cellular responses to time and environment.
